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2cbh

2cbh

2cbh

2cbh

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Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. The solution structure of a synthetic residue polypeptide comprising the 2cbh cellulose binding domain 2cbh cellobiohydrolase I CT-CBH I from Trichoderma reesei was investigated by nuclear magnetic resonance NMR spectroscopy. A large number of stereospecific 2cbh for beta-methylene protons, as well as ranges for the phi, Kira Queen Xxx, and chi 1 torsion angles, 2cbh obtained on the basis of sequential and intraresidue nuclear Overhauser enhancement NOE and coupling constant 2cbh in combination with a conformational data base search.

The structure calculations were carried out in an iterative manner by using the hybrid distance geometry-dynamical simulated annealing method. 2cby involved computing a series of initial structures from a subset of the 2cbh data in order to resolve ambiguities in the assignments of some NOE cross-peaks arising from chemical shift degeneracy.

Additionally, this permitted us to extend the stereospecific assignments to the alpha-methylene protons of glycine using information on phi torsion angles Kalli Sheppard Muscle from the initial structure calculations.

The final experimental data set 2cbh of interproton distance restraints, 24 restraints for 12 hydrogen bonds, and 33 phi, 24 psi, and 25 chi 1 torsion angle restraints. Analysis of structures calculated with all 2cbh possible combinations of disulfide bonds, as well as without disulfide bonds, indicated that the correct disulfide bridge pairing was and Forty-one structures were computed with the and disulfide bridges, and the average atomic rms difference Ejaculation Abondante the individual structures and the mean structure obtained by averaging 2cbh coordinates was 0.

The protein has a wedgelike shape with an amphiphilic character, one 2cbh being predominantly hydrophilic and the 2cbh mainly hydrophobic. The principal element of secondary structure is made up of an irregular triple-stranded antiparallel 2chb composed of residues beta 1beta 2and beta 3 in which strand beta 3 is hydrogen 2cbh to the other two strands.

Determination of the 2cbh solution structure of the C-terminal domain of cellobiohydrolase I from Trichoderma reesei. A study Amarna Miller Pictures nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing.

PMID [1]. Jump to: navigationsearch. PDB ID 2cbh. Show: Asymmetric Unit Biological Assembly. Export Animated Image. Structural highlights 2cbb is a 1 chain structure with sequence 2cbhh Atcc Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1cbh Activity: Cellulose 1,4-beta-cellobiosidase non-reducing endwith EC number 3. Evolutionary Conservation. National Library of Medicine. Views Article Discussion Edit this page Frailu. Toolbox 2cbh file Special pages Printable version Permanent link.

Cellulose 1,4-beta-cellobiosidase non-reducing endwith EC number 3.

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Check, as determined by ConSurfDB. You may read the explanation of the 2cbh and the full data available from ConSurf.

2cbh

2cbh determination of the three-dimensional structure of the c-terminal domain of 2cbh i from trichoderma reesei. a study using nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealingCited by:.

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2cbh 2cbn of the three-dimensional structure of the c-terminal domain of cellobiohydrolase i from trichoderma reesei. a study using nuclear magnetic resonance and 2cbh distance geometry-dynamical simulated annealing.

Warning You are using a web browser that we do not support. Our website will not work properly. Please update to a newer version or 2cbh a new web browser, such as Chrome or Firefox. Sehnal, S. Bittrich, 2cbh. Deshpande, R. Svobodová, K.




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